Marcussen H
Scand J Gastroenterol. 1978;13(3):277-81. doi: 10.3109/00365527809179820.
With the indirect immunofluorescence technique using sections of colon from guenon and rat, sera from patients with ulcerative colitis yielded positive staining of goblet cells. Eight sera, thus defined positive, two negative, and five from hospital controls, were applied to agar preparations of 12 different E. coli strains, normally found in human bacterial diseases and E. coli 0 14. With E. coli 0 group antigens, 2, 8 and 14 positive fluorescence reactions were regularly found in sera where positive immunofluorescence reaction aginst colon antigen could be demonstrated, indicating that antigen(s) from colon mucosa shares antigenic determinant(s) with some E. coli bacteria or bacterial components.
采用间接免疫荧光技术，使用来自非洲绿猴和大鼠的结肠切片，溃疡性结肠炎患者的血清对杯状细胞产生阳性染色。确定8份血清呈阳性，2份呈阴性，5份来自医院对照，将其应用于12种不同大肠杆菌菌株的琼脂制剂，这些菌株通常存在于人类细菌性疾病中以及大肠杆菌O14。对于大肠杆菌O群抗原，在可证明对结肠抗原呈阳性免疫荧光反应的血清中，经常发现2、8和14呈现阳性荧光反应，这表明结肠黏膜的抗原与某些大肠杆菌细菌或细菌成分具有共同的抗原决定簇。
