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产气气杆菌原生质体对甲型流感病毒的吸附作用。

Adsorption of influenza A virus by Aerobacter aerogenes spheroplasts.

作者信息

BROWN R J, BENEDICT A A, ARMSTRONG N

出版信息

J Bacteriol. 1962 May;83(5):1124-30. doi: 10.1128/jb.83.5.1124-1130.1962.

Abstract

Brown, Ronald J. (University of Kansas, Lawrence), Albert A. Benedict, and Nancy Armstrong. Adsorption of influenza A virus by Aerobacter aerogenes spheroplasts. J. Bacteriol. 83:1124-1130. 1962.-Aerobacter aerogenes spheroplasts adsorbed virus at a greater rate than whole A. aerogenes cells or cell-wall preparations. The largest fragments of sonic-disrupted spheroplasts were responsible for adsorption. Adsorption was characterized by rapid disappearance of viral hemagglutinin in about 8 min, elution of hemagglutinin, and followed again by complete adsorption of hemagglutinin. Viral activity was not recovered from spheroplasts after the final adsorption phase. Spheroplasts treated with heat, dilute periodate, or formaldehyde did not adsorb virus, and treatment of spheroplasts with receptor-destroying enzyme resulted in delayed adsorption.

摘要

布朗,罗纳德·J.(堪萨斯大学,劳伦斯),阿尔伯特·A.本尼迪克特,以及南希·阿姆斯特朗。产气气杆菌原生质体对甲型流感病毒的吸附。《细菌学杂志》83:1124 - 1130。1962年。——产气气杆菌原生质体吸附病毒的速率比产气气杆菌完整细胞或细胞壁制剂更快。经超声破碎的原生质体的最大片段负责吸附。吸附的特征是病毒血凝素在约8分钟内迅速消失,血凝素洗脱,随后血凝素再次完全吸附。在最终吸附阶段后,未从原生质体中回收病毒活性。经加热、稀高碘酸盐或甲醛处理的原生质体不吸附病毒,用受体破坏酶处理原生质体导致吸附延迟。

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