Ellerton H D, Collins L B, Gale J S, Yung A Y
Biophys Chem. 1976 Dec;6(1):47-57. doi: 10.1016/0301-4622(76)80060-9.
The hemocyanin from the crayfish Jasus edwardsii(=lalandii) has been studied using ultracentrifugation, viscosity, circular dichroism and oxygen binding techniques. Sedimentation velocity experiments at pH 7.0 indicated the presence of principal species with S 20w=16.4 S, and at higher pH the presence of a species with S20,w=5.2S. Sedimentation equilibrium experiments yielded molecular weights of 490 000 and 81 000 respectively, indicating that the larger unit is a hexamer of the monomer unit. However, preliminary experiments with gel filtration and electrophoresis under denaturing conditions indicate that more than one monomer species may be present with molecular weight in the range 76-100 000. Circular dichroism (CD) spectra are presented at pH 7.0,8.6,10.0 and 11.0 for oxy-, deoxy- and apo-hemocyanins. Slight differences were observed in the magnitude of the bands in the presence or absence of Mg++. Oxygen binding studies have been made at pH 6.1,7.0,8.8 and 10.6, in the presence of 0.01 M MgCl2. The extent of cooperative binding was indicated by a maximum value of n=3.7, and a pronounced bohr effect was observed.
利用超速离心、黏度、圆二色性和氧结合技术对小龙虾爱德华岩龙虾（=拉氏岩龙虾）的血蓝蛋白进行了研究。在pH 7.0条件下进行的沉降速度实验表明，主要存在沉降系数S20w = 16.4 S的组分，而在较高pH条件下，存在沉降系数S20,w = 5.2 S的组分。沉降平衡实验分别得出分子量为490 000和81 000，这表明较大的单元是单体单元的六聚体。然而，变性条件下的凝胶过滤和电泳初步实验表明，可能存在不止一种分子量在76 - 100 000范围内的单体组分。给出了氧合、脱氧和脱辅基血蓝蛋白在pH 7.0、8.6、10.0和11.0条件下的圆二色性（CD）光谱。在有或没有Mg++存在的情况下，观察到谱带强度存在细微差异。在0.01 M MgCl2存在的条件下，于pH 6.1、7.0、8.8和10.6进行了氧结合研究。协同结合程度由最大值n = 3.7表明，并且观察到明显的玻尔效应。
