Wilson T J, Davidson N J, Boyd R L, Gershwin M E
Department of Internal Medicine/Rheumatology, School of Medicine, University of California, Davis 95616.
Dev Immunol. 1992;2(1):19-27. doi: 10.1155/1992/32341.
The development of monoclonal antibodies (mAb) reactive with the thymic microenvironment has identified distinct subpopulations within the stromal component, but the function of these subregions in intrathymic T-cell differentiation remains essentially an enigma. In this study, we have used such a panel of mAb to examine the chicken thymus during ontogenic development to gain insight into the contributions of these thymic regions to the distinct phases of T-cell development and to further characterize the development of this organ. Our reagents have demonstrated the complex differentiation of the primitive endodermal epithelium into more specialized structures and the development of other thymic stromal components from mesectodermal cells. We also describe molecules localized to the subcapsular and perivascular regions, which have an ontogenic expression corresponding to the early localization and stimulation of thymic precursors and another molecule on the medullary vasculature expressed corresponding to the exit of mature cells from the thymus. In addition, two markers of distinct medullary epithelial clusters are initially expressed corresponding to the appearance of T-cell receptor-1 (TcR-1) and TcR-2 positive cells in the medulla, respectively. These mAb potentially represent excellent reagents for further definition of the thymic modulation of T-cell differentiation.
与胸腺微环境发生反应的单克隆抗体（mAb）的发展，已确定了基质成分中的不同亚群，但这些亚区域在胸腺内T细胞分化中的功能基本上仍是个谜。在本研究中，我们使用了这样一组单克隆抗体，在个体发育过程中对鸡胸腺进行检测，以深入了解这些胸腺区域对T细胞发育不同阶段的贡献，并进一步描述该器官的发育情况。我们的试剂已证明原始内胚层上皮向更特化结构的复杂分化，以及来自中胚层细胞的其他胸腺基质成分的发育。我们还描述了定位于被膜下和血管周围区域的分子，其个体发育表达与胸腺前体的早期定位和刺激相对应，以及髓质脉管系统上另一种分子的表达与成熟细胞从胸腺输出相对应。此外，两种不同髓质上皮簇的标志物最初分别在髓质中与T细胞受体-1（TcR-1）和TcR-2阳性细胞出现时表达。这些单克隆抗体可能是进一步界定胸腺对T细胞分化调节作用的优秀试剂。
