Hardy N M
Department of Pathology, University of Florida Health Science Center/Jacksonville 32209.
Ann Clin Lab Sci. 1992 Sep-Oct;22(5):286-9.
The severe complications of the acquired immunodeficiency syndrome represent the final phase of a prolonged course of immune system destruction during the infection by human immunodeficiency virus (HIV). Many of these complications can be predicted by measuring the depletion of CD4 positive lymphocytes. The CD4 positive lymphocyte counts are now widely accepted as a surrogate marker to assess the stage of disease and to determine immune response in major clinical trials. Other lymphocyte subsets are candidate surrogate markers for antiretroviral therapy. Our laboratory has utilized flow cytometry to perform lymphocyte subset testing, including CD4, CD8, CD4/CD8 ratio, and others for more than three years on persons with suspected immune deficiency. Results from our laboratory are presented to illustrate the use of these procedures in an urban, predominantly inner city population. The role of flow cytometry in monitoring patients with HIV infection is discussed.
获得性免疫缺陷综合征的严重并发症代表了人类免疫缺陷病毒（HIV）感染期间免疫系统长期破坏过程的最后阶段。通过测量CD4阳性淋巴细胞的耗竭情况，可以预测其中许多并发症。目前，CD4阳性淋巴细胞计数已被广泛用作评估疾病阶段和在主要临床试验中确定免疫反应的替代指标。其他淋巴细胞亚群是抗逆转录病毒治疗的候选替代指标。我们实验室利用流式细胞术对疑似免疫缺陷患者进行淋巴细胞亚群检测，包括CD4、CD8、CD4/CD8比值等，已有三年多时间。本文展示了我们实验室的结果，以说明这些检测方法在一个主要为市中心城区的城市人群中的应用情况。同时还讨论了流式细胞术在监测HIV感染患者中的作用。
