Watts M E, Woodcock M
Gray Laboratory Cancer Research Campaign, Mount Vernon Hospital, Northwood, Middlesex, U.K.
Eur J Cancer. 1992;28A(10):1628-32. doi: 10.1016/0959-8049(92)90056-8.
Flavone acetic acid (FAA) causes significant regression of larger established tumours in murine in vivo systems. This in vivo effect of FAA has been shown to include a vascular component. In an effort to elucidate the mechanism of action of FAA, we have studied the effects of FAA on the permeability of human endothelium in vitro. Monolayers of human umbilical vein endothelial cells (HUVEC) grown on polycarbonate filters were incubated in 1 mg/ml FAA for 120 min at 37 degrees C. During the first 60 min, there was a 6-8-fold increase in permeability; this was followed by a return to control levels even in the continued presence of FAA. In contrast, in the presence of tumour conditioned medium, FAA caused a rapid 6-fold increase in permeability which did not subsequently return to control levels. The permeability changes which occurred under the latter conditions were accompanied by a rapid contraction of the cytoskeleton. The permeability of monolayers of human melanoma cells was unaffected by FAA.
黄酮醋酸（FAA）可使小鼠体内已形成的较大肿瘤显著消退。FAA的这种体内效应已被证明包括血管成分。为了阐明FAA的作用机制，我们研究了FAA对体外人内皮细胞通透性的影响。将生长在聚碳酸酯滤膜上的人脐静脉内皮细胞（HUVEC）单层在37℃下于1mg/ml FAA中孵育120分钟。在最初的60分钟内，通透性增加了6 - 8倍；即使在持续存在FAA的情况下，随后也恢复到了对照水平。相比之下，在肿瘤条件培养基存在的情况下，FAA导致通透性迅速增加6倍，随后并未恢复到对照水平。在后一种条件下发生的通透性变化伴随着细胞骨架的快速收缩。人黑色素瘤细胞单层的通透性不受FAA影响。
