在微浴槽中对金鱼肠道进行P物质测定。
Assay of substance P on goldfish intestine in a microbath.
作者信息
GADDUM J H, SZERB J C
出版信息
Br J Pharmacol Chemother. 1961 Dec;17(3):451-63. doi: 10.1111/j.1476-5381.1961.tb01132.x.
Abstract
A technique is described for testing the effects of drugs on the intestine of goldfish in a microbath (0.05 ml.). This preparation may be used for the assay of substance P, which causes contractions of the muscle in doses as small as 1 m-u. Other substances in tissue extracts also act on this muscle, but the effects of most of these can be abolished by antagonists. Some tissues, however, contain an unknown substance which causes powerful contractions of the muscle and interferes with the assay of substance P. An attempt to demonstrate the release of substance P in the central nervous system was unsuccessful.
摘要
本文描述了一种在微浴槽（0.05毫升）中测试药物对金鱼肠道作用的技术。该制剂可用于检测P物质，P物质在低至1毫微克的剂量下就能引起肌肉收缩。组织提取物中的其他物质也作用于这种肌肉，但其中大多数物质的作用可被拮抗剂消除。然而，一些组织含有一种未知物质，它会引起肌肉强烈收缩并干扰P物质的检测。在中枢神经系统中证明P物质释放的尝试未成功。
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