Preudhomme C, Facon T, Zandecki M, Vanrumbeke M, Laï J L, Nataf E, Loucheux-Lefebvre M H, Kerckaert J P, Fenaux P
INSERM U 124, Institut de Recherches sur le Cancer de Lille, France.
Br J Haematol. 1992 Jul;81(3):440-3. doi: 10.1111/j.1365-2141.1992.tb08253.x.
We looked for mutations of exons 5-8 of the P53 gene in bone marrow cell from 37 cases of multiple myeloma, using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis and DNA sequencing. 25 patients also had cytogenetic analysis. A point mutation, leading to an amino acid change in the P53 protein was found in only one case, involving exon 5. These findings suggest that P53 mutations are very rare in multiple myeloma, and that this disease may be categorized among the few neoplasms where P53 abnormalities have very limited role, if any.
我们采用聚合酶链反应-单链构象多态性（PCR-SSCP）分析和DNA测序技术，对37例多发性骨髓瘤患者的骨髓细胞中P53基因外显子5-8的突变情况进行了研究。25例患者还进行了细胞遗传学分析。仅在1例患者中发现了1个导致P53蛋白氨基酸改变的点突变，该突变位于外显子5。这些研究结果表明，P53突变在多发性骨髓瘤中非常罕见，并且该疾病可能属于P53异常即便有作用也非常有限的少数肿瘤类型之一。
