Torres M J, Palomares J C
Departamento de Microbiología, Facultad de Medicina, Sevilla.
Enferm Infecc Microbiol Clin. 1992 Jun-Jul;10(6):345-8.
With arbitrary primer PCR technique it is possible to obtain amplification lane patterns easily and with good reproducibility from the genomic DNA of bacteria studied. There is also no need for prior information regarding the DNA sequence.
This method implies two cycles of amplification with low stringency, followed by a PCR of high stringency.
Using the above mentioned technique, we were able to show that Campylobacter spp from clinical samples could be separated as well as different strains from the same species.
According to our results as well as the ones from different authors applied to other microorganisms, we can assume that the method could be used in any bacterial species for epidemiologic studies purposes.
利用任意引物聚合酶链反应技术，能够轻松地从所研究细菌的基因组DNA中获得具有良好可重复性的扩增条带模式。而且无需有关DNA序列的先验信息。
该方法包括两个低严谨度的扩增循环，随后是一个高严谨度的聚合酶链反应。
使用上述技术，我们能够证明临床样本中的弯曲杆菌属菌种以及同一菌种的不同菌株均可被区分开来。
根据我们的结果以及其他作者应用于其他微生物的结果，我们可以假定该方法可用于任何细菌物种的流行病学研究。
