Abbolito M R, Ameglio F, Guerrera A M, Citarda F, Grassi A, Sciarretta F, Aceti A, Casale V, Gandolfo G M
Servizio di Analisi Cliniche, Istituto Regina Elena, Roma, Italy.
Ital J Gastroenterol. 1992 Sep;24(7):389-92.
Fifty-four gastric biopsies and their relative gastric juices were analyzed for the presence of Helicobacter pylori with both cultural and microscopic methods. Thirty-one samples were positive and twenty-three were negative. These data were therefore employed as references for the subsequent comparisons. Furthermore, the gastric juices were later tested to establish the urea concentration and the pH level. In addition, the sediment obtained after centrifugation was microscopically observed for the possible presence of other bacterial flora in the sample (unstained smears). The urease test on the bioptic specimens has also been evaluated. The presence of H pylori was strictly related to urea levels of less than 15 mg/dl and pH less than 3.5. Furthermore, H pylori was generally not associated with the presence of other bacterial flora (only 1 out of 12 samples). The latter instead, was almost exclusively present in high pH samples (with the exception of one). On the basis of these results, a simple diagnostic scheme was constructed to identify carrier subjects. All patients (14/14) with urea levels of more than 15 mg/dl were found to be negative as well as those presenting a pH of more than 3.5 (7/9) or evidence of other bacteria in the juices (8/9). The remaining subjects (30/31 or 29/31, respectively) presented H pylori in the gastric juices. The final classification was 96.3% (or 94.4%) correct.
采用培养和显微镜检查方法，对54份胃活检组织及其相应的胃液进行了幽门螺杆菌检测。31份样本呈阳性，23份呈阴性。因此，这些数据被用作后续比较的参考。此外，随后对胃液进行检测以确定尿素浓度和pH值。另外，对离心后获得的沉淀物进行显微镜观察，以检查样本中是否可能存在其他细菌菌群（未染色涂片）。还对活检标本进行了尿素酶试验评估。幽门螺杆菌的存在与尿素水平低于15mg/dl和pH值低于3.5密切相关。此外，幽门螺杆菌通常与其他细菌菌群的存在无关（12份样本中只有1份）。相反，其他细菌菌群几乎只存在于高pH值样本中（有一个样本除外）。基于这些结果，构建了一个简单的诊断方案来识别携带者。所有尿素水平高于15mg/dl的患者（14/14）以及pH值高于3.5的患者（7/9）或胃液中有其他细菌证据的患者（8/9）均被发现为阴性。其余受试者（分别为30/31或29/31）的胃液中存在幽门螺杆菌。最终分类的正确率为96.3%（或94.4%）。
