溶液中高分子量核糖核酸的结构。小角X射线散射研究。
The structure of high molecular weight ribonucleic acid in solution. A smallangle x-ray scattering study.
作者信息
TIMASHEFF S N, WITZ J, LUZZATI V
出版信息
Biophys J. 1961 Sep;1(7):525-37. doi: 10.1016/s0006-3495(61)86906-3.
Abstract
Small-angle x-ray scattering studies on an absolute scale have been carried out on isotropic solutions of high molecular weight RNA obtained from ascites tumor cells, E. coli, and yeast. It was found that in all three cases the RNA is composed of short rigid rods (50 to 150A in length) joined by small flexible regions. The rods account for almost the entire structure (at least 90 per cent); their radius of gyration about the axis and their mass per unit length are similar to those of DNA, suggesting a double-stranded helical structure. The rods are joined in an array forming the compact RNA molecule. On thermal degradation, the molecular superstructure disappears while the rods persist.
摘要
已经对从腹水肿瘤细胞、大肠杆菌和酵母中获得的高分子量RNA的各向同性溶液进行了绝对尺度的小角x射线散射研究。结果发现，在所有这三种情况下，RNA均由短的刚性棒（长度为50至150埃）通过小的柔性区域连接而成。这些棒几乎构成了整个结构（至少90%）；它们绕轴的回转半径和单位长度的质量与DNA相似，表明是双链螺旋结构。这些棒排列成阵列，形成紧密的RNA分子。热降解时，分子超结构消失，而棒状结构依然存在。
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2
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3
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4
A study of the alkaline hydrolysis of fractionated reticulocyte ribosomal ribonucleic acid and its relevance to secondary structure.
Biochem J. 1968 Feb;106(3):733-41. doi: 10.1042/bj1060733.
5
Sedimentation coefficients of RNA from 70S and 80S ribosomes.
Proc Natl Acad Sci U S A. 1967 Jan;57(1):164-9. doi: 10.1073/pnas.57.1.164.
6
A study of the hydrolysis of unfractionated reticulocyte ribosomal ribonucleic acid by pancreatic ribonuclease and its relevance to secondary structure.
Biochem J. 1967 May;103(2):431-52. doi: 10.1042/bj1030431.
本文引用的文献
1
SECONDARY STRUCTURE IN RIBONUCLEIC ACIDS.
Proc Natl Acad Sci U S A. 1959 Apr;45(4):482-99. doi: 10.1073/pnas.45.4.482.
2
SOME RELATIONS BETWEEN DNA AND RNA.
Proc Natl Acad Sci U S A. 1954 Aug;40(8):759-64. doi: 10.1073/pnas.40.8.759.
3
INTERACTION OF SMALL IONS AND MOLECULES WITH RIBONUCLEIC ACID.
Proc Natl Acad Sci U S A. 1961 Apr;47(4):465-9. doi: 10.1073/pnas.47.4.465.
4
THE ACCOMMODATION OF NONCOMPLEMENTARY BASES IN HELICAL POLYRIBONUCLEOTIDES AND DEOXYRIBONUCLEIC ACIDS.
Proc Natl Acad Sci U S A. 1960 Mar;46(3):311-21. doi: 10.1073/pnas.46.3.311.
5
Ribonucleic acid from Escherichia coli; preparation, characterization and physical properties.
Biochim Biophys Acta. 1959 Apr;32:320-37. doi: 10.1016/0006-3002(59)90604-3.
6
Light-scattering studies on ascites tumor cell RNA.
Biochim Biophys Acta. 1960 Jun 3;40:410-7. doi: 10.1016/0006-3002(60)91381-0.
7
[Structure of desoxyribonucleic acid in solution. Study by the diffusion of x-rays at small angles].
J Mol Biol. 1961 Apr;3:185-201. doi: 10.1016/s0022-2836(61)80045-4.
8
Base pairing in DNA.
J Mol Biol. 1960 Dec;2:363-71. doi: 10.1016/s0022-2836(60)80047-2.
9
The molecular weight of ribonucleic acid prepared from ascites-tumor cells.
Biochim Biophys Acta. 1958 Mar;27(3):662-3. doi: 10.1016/0006-3002(58)90412-8.
10
Preparation of nucleic acids from Ehrlich ascites tumor cells.
Science. 1956 Nov 30;124(3231):1077-8. doi: 10.1126/science.124.3231.1077.
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