大肠杆菌核糖体16S - RNA大RNA片段的构象。一项X射线和中子小角散射研究。
The conformation of a large RNA fragment from the E.coli ribosomal 16S-RNA. An X-ray and neutron small-angle scattering study.
作者信息
Osterberg R, Sjöberg B, Garrett G A, Müller R
出版信息
Nucleic Acids Res. 1980 Dec 20;8(24):6221-31. doi: 10.1093/nar/8.24.6221.
Abstract
A large 12S RNA fragment which constitutes the 5' two-thirds of 16S-RNA from the E. coli 30S subunit has been investigated by small-angle X-ray and neutron scattering. The results indicate that in reconstitution buffer the 12S-RNA fragment has a molecular weight of 270,000 +/- 20,000 and a radius of gyration of 7.1 nm. The scattering data are compatible with the RNA being folded into two major domains with the shapes of two adjacent, quite similar cylinders.
摘要
一个构成大肠杆菌30S亚基16S - RNA 5'三分之二的大12S RNA片段已通过小角X射线和中子散射进行了研究。结果表明，在重构缓冲液中，12S - RNA片段的分子量为270,000±20,000，回转半径为7.1纳米。散射数据与RNA折叠成两个主要结构域相符，这两个结构域的形状为两个相邻且非常相似的圆柱体。
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本文引用的文献
1
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Prog Biophys Mol Biol. 1963;13:105-73. doi: 10.1016/s0079-6107(63)80015-2.
2
Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence.细菌16S核糖体RNA的二级结构模型：系统发育、酶学及化学证据
Nucleic Acids Res. 1980 May 24;8(10):2275-93. doi: 10.1093/nar/8.10.2275.
3
Neutron scattering study of the 13 S fragment of 16 S RNA and its complex with ribosomal protein S4.16S RNA的13S片段及其与核糖体蛋白S4复合物的中子散射研究。
J Mol Biol. 1980 Feb 15;137(1):109-21. doi: 10.1016/0022-2836(80)90160-6.
4
On the conformation of phenylalanine specific transfer RNA. Studies on size and shape of the molecule by x-ray small angle scattering.关于苯丙氨酸特异性转移核糖核酸的构象。通过X射线小角散射对分子大小和形状的研究。
Eur J Biochem. 1970 Sep;15(3):401-9. doi: 10.1111/j.1432-1033.1970.tb01021.x.
5
Small angle x-ray scattering of a homogeneous gamm G-1 immunoglobulin.
Biochemistry. 1970 Jan 20;9(2):211-9. doi: 10.1021/bi00804a004.
6
X-ray scattering study of ribosomes from Escherichia coli.大肠杆菌核糖体的X射线散射研究。
J Mol Biol. 1969 Aug 28;44(1):89-102. doi: 10.1016/0022-2836(69)90406-9.
7
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J Mol Biol. 1974 Jun 25;86(2):411-32. doi: 10.1016/0022-2836(74)90028-x.
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