Jie W, Pei-Xi C
Department of Physiology, Sun Yat-Sen University of Medical Sciences, Guangzhou People's Republic of China.
Brain Res. 1992 May 29;581(2):269-72. doi: 10.1016/0006-8993(92)90717-n.
Experiments were performed in cats under chloralose anaesthesia and immobilized by Flaxedil. The discharge responses of cerebellar Purkinje cells (PC) were recorded with a microelectrode. The spontaneous activities of PC consisted of simple spike (SS) and complex spike (CS). When the saphenous nerve was stimulated at a low intensity, which elicited the A-fiber input only, the discharge responses (A-CED) consisted of an early component with short latency and late component with long latency in PC-SS and PC-CS. After A-fibers were blocked selectively by the polarizing current, the stimulation at the strength of C-fiber suprathreshold evoked the characteristic responses (C-CED) of PC-SS and PC-CS with middle latency. However, the C-CED could not be evoked by the inputs of A- and C-fibers simultaneously. These results suggested that the pure C-fiber input reaching the cerebellar PC passed through not only climbing fibers, but also mossy fibers, and elicited the characteristic responses (C-CED); these responses were neither the early component nor late component of the A-CED. When A- and C-fiber were activated at the same time, the C-CED might be inhibited by the A-fiber inputs.
实验在水合氯醛麻醉并用三碘季铵酚固定的猫身上进行。用微电极记录小脑浦肯野细胞（PC）的放电反应。PC的自发活动由简单锋电位（SS）和复合锋电位（CS）组成。当以低强度刺激隐神经，仅引出A纤维输入时，PC-SS和PC-CS中的放电反应（A-CED）由潜伏期短的早期成分和潜伏期长的晚期成分组成。在用极化电流选择性阻断A纤维后，以C纤维阈上强度进行刺激，可引出PC-SS和PC-CS具有中等潜伏期的特征性反应（C-CED）。然而，A和C纤维的输入同时作用时无法引出C-CED。这些结果表明，到达小脑PC的纯C纤维输入不仅通过攀缘纤维，还通过苔藓纤维，并引出特征性反应（C-CED）；这些反应既不是A-CED的早期成分也不是晚期成分。当A和C纤维同时激活时，C-CED可能会被A纤维输入抑制。
