PLAUT A G, FISHMAN W H
J Cell Biol. 1963 Feb;16(2):253-8. doi: 10.1083/jcb.16.2.253.
Androgens produced by stimulating mouse testis with gonadotropic hormones cause a rise in renal beta-glucuronidase but not an increase in acid or alkaline phosphatase. All subcellular components increase in beta-glucuronidase activity, with a relatively greater increment in particulate enzyme as compared with that free in the cytoplasm (non-sedimentable). A small percentage of recovered beta-glucuronidase, acid phosphatase, and alkaline phosphatase is found in material which rises to the surface during centrifugation in sucrose media (fraction I). The specific activity of beta-glucuronidase and acid phosphatase in this fraction is normally quite high with respect to the homogenate, while that of alkaline phosphatase is not. On the other hand, the fraction I material from androgen-stimulated mice exhibits a further increase in specific activity with respect to beta-glucuronidase and not acid phosphatase. It thus appears that there is an independence in the behavior of individual enzymes in response to physiologic stimuli in spite of obvious morphologic proximity.
用促性腺激素刺激小鼠睾丸产生的雄激素会导致肾脏β-葡萄糖醛酸酶升高，但不会使酸性或碱性磷酸酶增加。所有亚细胞成分的β-葡萄糖醛酸酶活性均增加，与细胞质中游离的（不可沉淀的）酶相比，颗粒酶的增加相对更大。在蔗糖介质中离心时升至表面的物质（组分I）中发现了一小部分回收的β-葡萄糖醛酸酶、酸性磷酸酶和碱性磷酸酶。相对于匀浆，该组分中β-葡萄糖醛酸酶和酸性磷酸酶的比活性通常相当高，而碱性磷酸酶则不然。另一方面，来自雄激素刺激小鼠的组分I物质相对于β-葡萄糖醛酸酶而非酸性磷酸酶，其比活性进一步增加。因此，尽管形态上明显接近，但似乎单个酶在对生理刺激的反应中行为具有独立性。
