Liang S M, Lee N, Chen Y Y, Liang C M
Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892.
Cell Immunol. 1992 Oct 1;144(1):131-42. doi: 10.1016/0008-8749(92)90231-d.
Internalization of IL-2 is important for its biological activities. The internalization of IL-2 was regulated by the duration of glutathione (GSH) treatment in CTLL-2 and CT-4R cells. Flow cytometric studies showed that the level of surface IL-2 receptors was not increased by GSH treatment. Northern blot analysis showed that the mRNA of IL-2Rp55 and IL-2Rp70, the two major components of the high-affinity IL-2 receptors, was increased 6 hr after GSH treatment. The appearance rate of membrane IL-2 receptors in GSH-treated cells was faster than that of the untreated cells. GSH also shortened the half-life (from 5 to less than or equal to 3 hr) and thus increased the turnover of the surface high-affinity IL-2 receptors. These results suggest that although GSH does not affect the level of surface IL-2 receptors, GSH may regulate the internalization of IL-2 by enhancing the synthesis and turnover of surface IL-2 receptors.
白细胞介素-2（IL-2）的内化作用对其生物学活性很重要。在CTLL-2和CT-4R细胞中，IL-2的内化作用受谷胱甘肽（GSH）处理时间的调控。流式细胞术研究表明，GSH处理并未使表面IL-2受体水平升高。Northern印迹分析显示，高亲和力IL-2受体的两个主要成分IL-2Rp55和IL-2Rp70的mRNA在GSH处理6小时后增加。GSH处理细胞中膜IL-2受体的出现速率比未处理细胞更快。GSH还缩短了半衰期（从5小时缩短至小于或等于3小时），从而加快了表面高亲和力IL-2受体的周转。这些结果表明，尽管GSH不影响表面IL-2受体的水平，但GSH可能通过增强表面IL-2受体的合成和周转来调节IL-2的内化作用。
