纤细裸藻中的微粒体乙醇氧化系统。裸藻与哺乳动物细胞系统之间的相似性。 - Suppr | 超能文献

纤细裸藻中的微粒体乙醇氧化系统。裸藻与哺乳动物细胞系统之间的相似性。

Microsomal ethanol-oxidizing system in Euglena gracilis. Similarities between Euglena and mammalian cell systems.

Julistiono H, Briand J

Laboratoire des Membranes Biologiques, Université Paris, France.

Comp Biochem Physiol B. 1992 Aug;102(4):747-55. doi: 10.1016/0305-0491(92)90074-2.

Abstract

ADH activity of Euglena grown with 50 mM ethanol decreased, but MEOS activity increased with a corresponding increase in the total amount of cytochrome P-450. 2. Phenobarbital treatment increased the total amount of cytochrome P-450. 3. CO and KCN, cytochrome P-450 ligands, diminished acetaldehyde formed from ethanol oxidation by MEOS. 4. The amounts of NAD(P)H cytochrome c reductases and cytochrome b5 type, components of microsomal monooxygenase reaction, have been spectrophotometrically measured. 5. NAD(P)H cytochrome c reductases activities were induced by phenobarbital. 6. DMSO, an inhibitor of rabbit MEOS, inhibited O2 consumption (11-20%) by Euglena grown with an ethanol, but not a lactate medium. 7. These studies indicate the presence of cytochrome P-450-dependent MEOS in Euglena similar to that in the mammalian hepatic cell.

摘要

在含50 mM乙醇的条件下培养的眼虫的抗利尿激素（ADH）活性降低，但微粒体乙醇氧化系统（MEOS）活性增加，同时细胞色素P - 450总量相应增加。2. 苯巴比妥处理增加了细胞色素P - 450的总量。3. 细胞色素P - 450配体一氧化碳（CO）和氰化钾（KCN）减少了由MEOS将乙醇氧化形成的乙醛。4. 已通过分光光度法测定了微粒体单加氧酶反应的组分烟酰胺腺嘌呤二核苷酸（磷酸）-细胞色素c还原酶和细胞色素b5的含量。5. 苯巴比妥诱导了烟酰胺腺嘌呤二核苷酸（磷酸）-细胞色素c还原酶的活性。6. 二甲亚砜（DMSO）是兔MEOS的抑制剂，它抑制在含乙醇而非乳酸培养基中培养的眼虫的耗氧量（11 - 20%）。7. 这些研究表明眼虫中存在与哺乳动物肝细胞中类似的细胞色素P - 450依赖性MEOS。