Thuillier-Bruston F, Julistiono H, Briand J
Laboratoires Biocodex, Montrouge, France.
Biochem Med Metab Biol. 1991 Apr;45(2):263-9. doi: 10.1016/0885-4505(91)90030-o.
This study indicates for the first time the presence of cytochrome P450 in the microsomes of Euglena grown in lactate medium and substantiates the use of Euglena as a hepatic cell model. Similar effects of ethanol on Euglena and on rat hepatic microsomes were demonstrated: (i) decrements in the quantities of FA per milligram of proteins; (ii) increases in the proportions of PE; (iii) decreases in the proportions of PC; and (iv) production of cytochrome P450, degraded in P420. The citrulline-malate reestablishes in the microsomes the phospholipid environment and the cytochrome P450 concentration. These findings illustrate that the complex acts on the lipid peroxidation via the changes in cytochrome P450 activity.
本研究首次表明，在乳酸培养基中生长的眼虫微粒体中存在细胞色素P450，并证实了眼虫作为肝细胞模型的用途。乙醇对眼虫和大鼠肝微粒体具有相似的作用：(i) 每毫克蛋白质中脂肪酸（FA）含量减少；(ii) 磷脂酰乙醇胺（PE）比例增加；(iii) 磷脂酰胆碱（PC）比例降低；(iv) 细胞色素P450生成，在P420中降解。瓜氨酸 - 苹果酸可在微粒体中重建磷脂环境和细胞色素P450浓度。这些发现表明，该复合物通过细胞色素P（450）活性的变化对脂质过氧化起作用。
