Onténiente B, Nothias F, Geffard M, Peschanski M
Groupe de Recherche de Médecine Nucléaire, INSERM CHU Henri Mondor, Créteil, France.
Dev Neurosci. 1992;14(2):130-43. doi: 10.1159/000111657.
This work was undertaken to determine whether or not cell suspension transplants provide a promising vehicle for the in vivo study of neuronal development. Cell suspensions of rat fetal tissue that contained both reticular (RT) and ventrobasal primordia were transplanted into the excitotoxically lesioned somatosensory thalamus of adult rats. The fine structure of transplanted GABAergic RT neurons that were identified by immunocytochemistry was investigated 5 days to 7 months following transplantation. The dissociation and transplantation of the tissue results in disruption of the extracellular matrix and alteration of cellular interactions which could introduce major changes in neuronal maturation. Our results show that maturation of transplanted GABAergic RT neurons was comparable to normal and that they were able to establish and receive, with one exception, usual sets of connections. In contrast, disruption of the tissue was responsible for the definitive loss of organotypic characteristics and the emergence of abnormal connections, especially synaptic connections from somatosensory afferents, the functional significance of which will be important to determine.
开展这项工作是为了确定细胞悬浮移植是否为神经元发育的体内研究提供了一种有前景的载体。将含有网状（RT）和腹侧基底原基的大鼠胎儿组织细胞悬液移植到成年大鼠经兴奋性毒素损伤的体感丘脑。在移植后5天至7个月，研究了通过免疫细胞化学鉴定的移植GABA能RT神经元的精细结构。组织的解离和移植导致细胞外基质的破坏和细胞相互作用的改变，这可能会引起神经元成熟的重大变化。我们的结果表明，移植的GABA能RT神经元的成熟与正常情况相当，并且除了一个例外，它们能够建立并接受通常的连接组。相比之下，组织的破坏导致器官型特征的明确丧失和异常连接的出现，尤其是体感传入神经的突触连接，其功能意义有待确定。
