Quantitative immunological determination of brush-border protein in urine. Their role in the progression of inflammatory and toxic renal damage.

In urine concentrates form patients with acute and chronic glomerulonephritis and patients under cytostatic treatment, remarkable amounts of membrane-bound alanine-aminopeptidase, alkaline-phosphatase and gamma-glutamyltraspeptidase could be recognized. With an anti-brush-border antisera membrane-bound enzymes could be differentiated from soluble fractions of other origins. Patients with recognizable membrane proteins in urine showed tendencies toward progression of their kidney diseases. Patients under cytostatic treatment with high dose showed a very early output of these enzymes while others under low dose started after a longer treatment with the excretion of membrane-bound enzymes.