有丝分裂器。分离单元的精细结构。
The mitotic apparatus. Fine structure of the isolated unit.
作者信息
KANE R E
出版信息
J Cell Biol. 1962 Nov;15(2):279-87. doi: 10.1083/jcb.15.2.279.
Abstract
The fine structure of the mitotic apparatus isolated from the sea urchin egg has been investigated. The isolation was accomplished by lysis of metaphase eggs in a 1 M solution of hexanediol, buffered at pH 6. The fine structure of the isolated apparatus was studied after fixation with osmium tetroxide directly in the isolation medium. The spindle is composed of fine fibrils, approximately 20 mmicro in diameter, which appear tubular. Similar fibrils, radially oriented, are found in the aster. If the isolated mitotic apparatus is exposed to water at pH 6 before fixation, the structure is considerably modified. The most pronounced effects are an increase in the number of large membrane-bounded vesicles and in the amount of free granular material present. The conditions necessary for the fixation of the mitotic apparatus in dividing cells are discussed in the light of these observations on the isolated unit.
摘要
对从海胆卵中分离出的有丝分裂器的精细结构进行了研究。分离过程是通过在pH 6缓冲的1 M己二醇溶液中裂解中期卵来完成的。在直接于分离介质中用四氧化锇固定后，研究了分离出的有丝分裂器的精细结构。纺锤体由直径约20微米的细纤维组成，这些纤维呈管状。在星体中也发现了呈放射状排列的类似纤维。如果在固定前将分离出的有丝分裂器暴露于pH 6的水中，其结构会发生显著改变。最明显的影响是有膜大泡数量的增加以及游离颗粒物质数量的增加。根据对分离单元的这些观察结果，讨论了在分裂细胞中固定有丝分裂器所需的条件。
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