Kubota Y, Sato H, Takahashi S
Division of Comparative Radiotoxicology, National Institute of Radiological Sciences, Chiba, Japan.
Environ Health Perspect. 1992 Jul;97:163-5. doi: 10.1289/ehp.9297163.
The effect of external gamma-irradiation on the survival of alveolar macrophages (AM) and on the extracellular release of 59Fe from AM-ingested [59Fe]iron hydroxide colloid was investigated in vitro using cells from C3H mice and Wistar rats. 59Fe release from mouse AM was enhanced by irradiation in a dose-dependent fashion, but irradiation up to 112 Gy did not affect the release from rat AM. Cell survival of AM, measured by direct counting of cell nuclei of adherent AM or vital staining with crystal violet, decreased dose dependently in mice and rats, but mouse AM were more radiosensitive than rat AM.
利用C3H小鼠和Wistar大鼠的细胞，在体外研究了外部γ射线照射对肺泡巨噬细胞（AM）存活以及AM摄取的[59Fe]氢氧化铁胶体中59Fe细胞外释放的影响。小鼠AM的59Fe释放以剂量依赖性方式因照射而增强，但高达112 Gy的照射对大鼠AM的释放没有影响。通过直接计数贴壁AM的细胞核或用结晶紫进行活细胞染色来测量的AM细胞存活率，在小鼠和大鼠中均呈剂量依赖性降低，但小鼠AM比大鼠AM对辐射更敏感。
