BIBB W R, STRAUGHN W R
J Bacteriol. 1962 Nov;84(5):1094-8. doi: 10.1128/jb.84.5.1094-1098.1962.
Bibb, William R. (University of North Carolina, Chapel Hill) and W. R. Straughn. Formation of protoplasts from Streptococcus faecalis by lysozyme. J. Bacteriol. 84:1094-1098. 1962.-Incubation of whole cells of Streptococcus faecalis F24 in the presence of the crystalline egg-white lysozyme and appropriate sucrose concentration resulted in the formation of discrete spherical structures. On dilution, these osmotically fragile structures lysed immediately. Methyl pentose determinations on isolated cell walls and protoplast membranes verified the presence of rhamnose in the cell walls and its essentially complete absence in protoplast membranes. Cell walls were rendered soluble by lysozyme. After lysozyme treatment of cell walls, 96% of the rhamnose present was not sedimented by centrifugation at 12,500 x g for 30 min. No cell-wall structures were recognized by phasecontrast or electron microscopy. After direct lysis of whole cells of S. faecalis F24 by lysozyme, protoplast membranes were isolated. It is concluded that, in the strain of group D streptococcus studied, lysozyme effectively removes the cell wall.
比布，威廉·R.（北卡罗来纳大学教堂山分校）和W.R.斯特劳恩。溶菌酶作用下粪肠球菌原生质体的形成。《细菌学杂志》84:1094 - 1098。1962年。——在结晶蛋清溶菌酶和合适的蔗糖浓度存在的情况下，对粪肠球菌F24全细胞进行孵育，导致形成离散的球形结构。稀释后，这些渗透压敏感的结构立即裂解。对分离的细胞壁和原生质体膜进行甲基戊糖测定，证实细胞壁中存在鼠李糖，而原生质体膜中基本不存在。溶菌酶使细胞壁溶解。用溶菌酶处理细胞壁后，96%的存在的鼠李糖在12,500×g离心30分钟后未沉淀。相差显微镜或电子显微镜均未识别到细胞壁结构。通过溶菌酶直接裂解粪肠球菌F24全细胞后，分离出原生质体膜。得出的结论是，在所研究的D组链球菌菌株中，溶菌酶能有效去除细胞壁。
