Lew R R, Garrill A, Covic L, Heath I B, Serlin B S
York University, Department of Biology, Ont., Canada.
FEBS Lett. 1992 Oct 5;310(3):219-22. doi: 10.1016/0014-5793(92)81336-k.
Protoplasts of the filamentous alga, Mougeotia, and the filamentous fungal oomycete, Saprolegnia ferax, exhibit two K+ ion channels (2-6 pA) using the patch-clamp technique when the seals are less than 1 G omega (about 100 M omega). The membrane potential of the protoplasts was near 0 mV as measured intracellularly with double-barreled micropipettes; thus, inward K+ flux is due solely to concentration differences. Although conductances are in the range expected for K+ channels, the activity at 0 mV is not seen in other organisms under gigaseal conditions. This paper draws attention to the usefulness of this subsidiary patch-clamp technique and the novel characteristics of ion channels in Mougeotia and Saprolegnia.
丝状藻类穆氏藻（Mougeotia）和丝状真菌性卵菌水霉（Saprolegnia ferax）的原生质体，当封接电阻小于1 GΩ（约100 MΩ）时，采用膜片钳技术可显示出两种钾离子通道（2 - 6 pA）。用双管微电极在细胞内测量时，原生质体的膜电位接近0 mV；因此，钾离子内流完全是由浓度差异引起的。尽管电导处于钾离子通道预期的范围内，但在千兆封接条件下，其他生物体在0 mV时并未观察到这种活性。本文提请注意这种辅助膜片钳技术的实用性以及穆氏藻和水霉中离子通道的新特性。
