RABINOVITCH M, PLAUT W
J Cell Biol. 1962 Dec;15(3):535-40. doi: 10.1083/jcb.15.3.535.
Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae contained around 11,000 particles. Amebae from two other strains contained similar particles. The particles were found to be clustered in fasted cells and redispersed after feeding. A marked increase in the particle population was noted in anucleate fragments. These results, together with those previously presented, suggest that the particles multiply intracellularly. Their nature and their relationship to previous work on nucleic acid labeling in Amoeba are discussed.
在用吖啶橙染色后，对大变形虫细胞质中含核酸的颗粒进行了计数（参见参考文献1）。发现每个变形虫的颗粒数量与细胞年龄和大小相关。新生的子细胞平均颗粒数为5400个，而即将分裂的变形虫含有约11,000个颗粒。另外两个菌株的变形虫也含有类似的颗粒。这些颗粒在饥饿的细胞中聚集，进食后重新分散。在无核碎片中观察到颗粒数量显著增加。这些结果与之前呈现的结果一起表明，这些颗粒在细胞内增殖。文中讨论了它们的性质以及它们与之前关于变形虫核酸标记研究的关系。
