Paveto C, Montero L, Passeron S
Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
FEBS Lett. 1992 Oct 12;311(1):51-4. doi: 10.1016/0014-5793(92)81365-s.
GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a membrane protein of 40 kDa. Pertussis toxin catalyzes the transference of the radioactivity from [32P]NAD to a 32 kDa protein. Two major proteins of 40-42 and 30-32 kDa can also be recognized in Western blots by an anti G alpha-common antibody. The results support the idea that G proteins are part of the hormone sensory transduction chain of Candida [(1990) Biochem. Biophys. Res. Commun. 167, 1177-1183].
通过用霍乱毒素、NAD和ATP对膜蛋白进行预孵育，二态性致病真菌白色念珠菌的腺苷酸环化酶的GTP刺激作用大大增强。在[32P]NAD存在的情况下，毒素催化放射性共价掺入40 kDa的膜蛋白中。百日咳毒素催化放射性从[32P]NAD转移到32 kDa的蛋白质中。抗Gα通用抗体在蛋白质免疫印迹中也可识别出40 - 42 kDa和30 - 32 kDa的两种主要蛋白质。这些结果支持了G蛋白是白色念珠菌激素传感转导链一部分的观点[（1990年）《生物化学与生物物理研究通讯》167，1177 - 1183]。
