ROSENBERG M D
J Cell Biol. 1963 May;17(2):289-97. doi: 10.1083/jcb.17.2.289.
Observations have been made on the response, in vitro, of cultured and freshly dissociated cells to mechanical deformation. Large numbers of individual cells were studied by means of a special culture chamber bounded by two parallel glass coverslips whose spacing could be reduced from 140 to 2 microns in steps of roughly 0.5 micron. The degree of deformation required for herniation of the cell surface was measured. These measurements lead to the definition of a statistical index characteristic of the extensibility of cell surfaces. This index has been shown to be distinctive for several types of cells; to alter with certain stages of embryonic development; and to be stable with respect to the culturing of cells and certain alterations in the method of cell culture.
已对培养的和刚解离的细胞在体外对机械变形的反应进行了观察。借助一个特殊的培养室对大量单个细胞进行了研究,该培养室由两个平行的玻璃盖玻片界定,其间距可从140微米逐步减小到2微米,步长约为0.5微米。测量了细胞表面疝出所需的变形程度。这些测量结果得出了一个细胞表面可伸展性的统计指标特征。已证明该指标对几种类型的细胞具有独特性;随胚胎发育的某些阶段而变化;并且对于细胞培养和细胞培养方法的某些改变而言是稳定的。