Schiechl H
Z Naturforsch C Biosci. 1977 Jan-Feb;32(1-2):67-71.
The paper describes a method for the large-scale isolation of protein III (protein E, major intrinsic protein) from the human erythrocyte membrane with little expenditure of time. By treatment of the erythrocyte ghosts with deluted HCl at pH 2.3 and 0 degrees C some membrane proteins can be extracted. From the remaining "rest" membranes, whose major constituents, besides phospholipides, are protein PAS-1, other carbohydrate containing proteins and protein III, the latter can be separated in pure form by means of gel filtration. The paper reports on the amount, purity and possible structural modifications of the protein III obtained by this isolation method.
本文描述了一种能在短时间内从人红细胞膜大规模分离蛋白质III（蛋白质E，主要内在蛋白）的方法。通过在pH 2.3和0℃下用稀释的HCl处理红细胞血影，可提取一些膜蛋白。在剩余的“残余”膜中，除磷脂外，主要成分是蛋白质PAS-1、其他含碳水化合物的蛋白质和蛋白质III，通过凝胶过滤可将后者分离成纯品。本文报道了通过这种分离方法获得的蛋白质III的量、纯度及可能的结构修饰。
