淋巴细胞增殖的测量：放射性和光度法的批判性分析。
Measurement of lymphocyte proliferation: critical analysis of radioactive and photometric methods.
作者信息
Wemme H, Pfeifer S, Heck R, Müller-Quernheim J
机构信息
Third Department of Internal Medicine, Johannes Gutenberg University, Mainz, Germany.
出版信息
Immunobiology. 1992 Jun;185(1):78-89. doi: 10.1016/S0171-2985(11)80319-0.
Different methods of lymphocyte proliferation are compared to identify a non-radioactive alternative to 3H-thymidine-test. The enzymatic assays evaluating the turnover of mitochondrial dehydrogenases (MTT-test) and lysosomal hexosaminidase (NAG-test) proved not sensitive enough to substitute for 3H-thymidine incorporation. The incorporation of the nucleotide analog 5-bromodeoxyuridine (BrdU) can be exploited using an ELISA-system (enzyme linked immunosorbent assay) employing a monoclonal anti-BrdU antibody to measure cell proliferation. An optimized test protocol of the BrdU-ELISA which fulfills the requirements for a sensitive and practicable non-radioactive alternative to 3H-thymidine-test is presented.
比较了不同的淋巴细胞增殖方法，以确定一种替代³H-胸腺嘧啶核苷检测的非放射性方法。评估线粒体脱氢酶周转率的酶法检测（MTT检测）和溶酶体己糖胺酶（NAG检测）被证明不够灵敏，无法替代³H-胸腺嘧啶核苷掺入法。可以利用核苷酸类似物5-溴脱氧尿苷（BrdU）的掺入，采用酶联免疫吸附测定（ELISA）系统，使用单克隆抗BrdU抗体来测量细胞增殖。本文介绍了一种优化的BrdU-ELISA检测方案，该方案满足了作为³H-胸腺嘧啶核苷检测灵敏且实用的非放射性替代方法的要求。
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