Hardard'ottir I, Whelan J, Kinsella J E
Lipids Research Laboratory, Cornell University, Ithaca, New York.
Immunology. 1992 Aug;76(4):572-7.
Cell-associated and secreted tumour necrosis factor (TNF), prostaglandin (PG) E2, and 6-keto PGF1 alpha were monitored at various times following in vitro stimulation of resident peritoneal macrophages with lipopolysaccharide (LPS). Macrophages were obtained from mice maintained on diets containing 1.5 wt% n-3 polyunsaturated fatty acids (PUFA)+ 1.5 wt% n-6 fatty acids; 1.5 wt% n-6 fatty acids; or 3 wt% n-6 fatty acids, for 4 weeks. Cell-associated TNF increased transiently in the resident peritoneal macrophages from mice consuming all diets and decreased after TNF secretion had reached maximum and plateaued. Macrophages from mice consuming the n-3 PUFA contained more cell-associated TNF and secreted more TNF than macrophages from mice consuming diets containing n-6 fatty acids only, at all time-points studied. Macrophages from mice consuming the n-3 PUFA showed an earlier increase in cell-associated and secreted TNF compared with macrophages from mice consuming n-6 fatty acids only. Kinetics of maximum TNF production was not affected by the diets and dietary n-3 PUFA did not cause a prolonged increase in TNF secretion. Macrophages from mice consuming the n-3 PUFA produced less PG than macrophages from mice consuming the n-6 fatty acids only. PG secretion increased following appearance of cell-associated TNF but when PG had accumulated in the medium there was no further increase in TNF production.
在用脂多糖（LPS）体外刺激驻留腹膜巨噬细胞后的不同时间，监测细胞相关和分泌的肿瘤坏死因子（TNF）、前列腺素（PG）E2和6-酮PGF1α。巨噬细胞取自喂食含1.5 wt% n-3多不饱和脂肪酸（PUFA）+ 1.5 wt% n-6脂肪酸、1.5 wt% n-6脂肪酸或3 wt% n-6脂肪酸饮食4周的小鼠。食用所有饮食的小鼠的驻留腹膜巨噬细胞中，细胞相关TNF短暂增加，在TNF分泌达到最大值并趋于平稳后下降。在所有研究的时间点，食用n-3 PUFA的小鼠的巨噬细胞比仅食用含n-6脂肪酸饮食的小鼠的巨噬细胞含有更多的细胞相关TNF，且分泌更多的TNF。与仅食用n-6脂肪酸的小鼠的巨噬细胞相比，食用n-3 PUFA的小鼠的巨噬细胞中细胞相关和分泌的TNF增加得更早。最大TNF产生的动力学不受饮食影响，饮食中的n-3 PUFA也不会导致TNF分泌的长期增加。食用n-3 PUFA的小鼠的巨噬细胞产生的PG比仅食用n-6脂肪酸的小鼠的巨噬细胞产生的少。PG分泌在细胞相关TNF出现后增加，但当PG在培养基中积累时，TNF产生不再进一步增加。
