Gelbart A, Goldman R H
Biochim Biophys Acta. 1977 Apr 12;481(2):689-94. doi: 10.1016/0005-2744(77)90302-3.
ATP plus Mg2+ plus Na+ supported [3H]ouabain binding to canine left ventricular tissue homogenates and microsomal (Na+ + K+)-ATPase (ATP phosphohydrolase, EC 3.6.1.3) activity from the same tissue were measured. A linear relationship was found between the initial velocity of [3H]ouabain binding to tissue homogenates and microsomal (Na+ + K+)-ATPase activity from the same tissue in the presence and absence of in vivo bound digoxin. In vivo bound digoxin reduced both measurements. With tissue from digoxin-free hearts, a linear relationship was also obtained between the initial velocity and the maximum level of [3H]ouabain binding to tissue homogenate. Binding of [3H]ouabain to whole tissue homogenate is a convenient method for estimating (Na+ + K+)-ATPase activity in small left ventricular biopsy samples.
测定了ATP加Mg2+加Na+对犬左心室组织匀浆中[3H]哇巴因结合的支持作用以及来自同一组织的微粒体（Na+ + K+）-ATP酶（ATP磷酸水解酶，EC 3.6.1.3）活性。在有和没有体内结合地高辛的情况下，发现[3H]哇巴因与组织匀浆的结合初速度和来自同一组织的微粒体（Na+ + K+）-ATP酶活性之间存在线性关系。体内结合的地高辛降低了这两项测量值。对于来自无地高辛心脏的组织，[3H]哇巴因与组织匀浆结合的初速度和最大水平之间也获得了线性关系。[3H]哇巴因与全组织匀浆的结合是估计小的左心室活检样本中（Na+ + K+）-ATP酶活性的一种便捷方法。
