Baskova I P, Memon M S, Romanov V V, Rusak A F
Biokhimiia. 1977 Jan;42(1):95-9.
A process of spontaneous activation of bovine prothrombin under acylation of cytacone anhydride is studied by means of ring dichroism and disc electrophoresis in sodium dodecylsulphate. Ring dichroism data have shown that native prothrombin contains 14% of alpha-helical structures, 36% of beta-structures and 50% of random coil. Cytraconylation results in a fragmentation of native prothrombin chain and is accompanies by the decrease of alpha-helical regions in the fragments formed. Poly-L-lysine, modified by citracone anydride, does not form alpha-helical regions.
通过在十二烷基硫酸钠中采用圆二色性和圆盘电泳法，研究了在环戊烯酸酐酰化作用下牛凝血酶原的自发激活过程。圆二色性数据表明，天然凝血酶原含有14%的α-螺旋结构、36%的β-结构和50%的无规卷曲。环戊烯酰化导致天然凝血酶原链断裂，并伴随着所形成片段中α-螺旋区域的减少。经环戊烯酸酐修饰的聚-L-赖氨酸不形成α-螺旋区域。
