Pouyet J C
C R Seances Soc Biol Fil. 1976;170(5):942-5.
The tail of the Alytes obstetricans tadpole, isolated at different stages (end of proclimax and climax), was studied in organ culture. The addition of thyroxine at a concentration of 5.10(-7) induces an involution slower than in vivo. Besides, this regression is comparatively slower in similar conditions, than in Xenopus laevis. This delay could be explained by the important volume and the very developed musculature of this anuran tail. Ultrastructural controls reveal the form of the muscles, the neural tube and the phagocytic cells in the tail metamorphising in vitro.
对处于不同阶段(前高峰期末期和高峰期)分离出的产婆蟾蝌蚪的尾巴进行了器官培养研究。添加浓度为5×10⁻⁷ 的甲状腺素会诱导其退化,且比在体内时更慢。此外,在类似条件下,这种退化比非洲爪蟾的退化相对更慢。这种延迟可能是由于这种无尾目动物尾巴的体积较大且肌肉组织非常发达。超微结构对照揭示了体外变态过程中尾巴里肌肉、神经管和吞噬细胞的形态。