Separation of theophylline and its analogues by micellar electrokinetic chromatography: application to the determination of theophylline in human plasma.

Micellar electrokinetic chromatographic separation of theophylline and its analogues was investigated using sodium dodecyl sulphate (SDS) as a micellar phase. The effects of pH, micelle concentration, applied voltage and temperature on the separation and preliminary quantitative analysis were studied for the determination of theophylline in human plasma. The data indicate that this technique could be used as the reference or routine method of theophylline measurement in therapeutic drug monitoring.