Teffera Y, Baird W M, Smith D L
Department of Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, IN 47907.
J Chromatogr. 1992 May 20;577(1):69-76. doi: 10.1016/0378-4347(92)80599-l.
An improved method is described for detecting glucuronide and sulfate conjugates of benzo[a]pyrene in medium from cell cultures treated with benzo[a]pyrene. This method is based on a microbore high-performance liquid chromatograph directly coupled to a high-resolution continuous-flow fast atom bombardment mass spectrometer. Sulfate and glucuronide conjugates, as well as some structural isomers of glucuronide conjugates, were fully separated by the reversed-phase microbore high-performance liquid chromatography conditions used in this study. Since the method does not rely on the use of radiolabeled materials, it may be used to detect conjugates of a wide variety of hydrocarbons. The high sensitivity and selectivity of the method were demonstrated by detecting conjugates in the media of cell cultures derived from mice, hamsters and humans.
描述了一种改进的方法，用于检测用苯并[a]芘处理的细胞培养基中苯并[a]芘的葡萄糖醛酸苷和硫酸酯共轭物。该方法基于直接连接到高分辨率连续流快原子轰击质谱仪的微径高效液相色谱仪。本研究中使用的反相微径高效液相色谱条件可完全分离硫酸酯和葡萄糖醛酸苷共轭物，以及葡萄糖醛酸苷共轭物的一些结构异构体。由于该方法不依赖于使用放射性标记物质，因此可用于检测多种碳氢化合物的共轭物。通过检测来自小鼠、仓鼠和人类的细胞培养基中的共轭物，证明了该方法的高灵敏度和选择性。
