Ariga S, Dukelow W R
Fertil Steril. 1977 May;28(5):577-80. doi: 10.1016/s0015-0282(16)42560-4.
A laparoscopic uterine flushing technique has been developed for the recovery of preimplantation embryos from the squirrel monkey. Fourteen adult female squirrel monkeys were induced to ovulate with 4 or 5 days' treatment with follicle-stimulating hormone (1 mg) followed by human chorionic gonadotropin (HCG) (500 IU). Natural mating with a fertile male or artificial insemination was performed near the time of ovulation. Thirty-six hours and eleven days after HCG administration, the females were laparoscoped to determine whether ovulation had occurred. Under laparoscopic control, 1.2 to 3 ml of warmed saline were flushed through the uterine lumen 4 to 7 and 15 to 17 days after HCG administration. Flushed fluid was recovered with a plyethylene catheter or a Pasteur pipette inserted into the cleaned vagina. Fifty-eight uterine flushings were performed, with an average of 65.4% fluid recovery. Six nonfertilized ova and two preimplantation blastocysts were recovered by this technique.
已开发出一种腹腔镜子宫冲洗技术，用于从松鼠猴体内回收植入前胚胎。对14只成年雌性松鼠猴进行4或5天的促卵泡激素（1毫克）治疗，随后注射人绒毛膜促性腺激素（HCG）（500国际单位），诱导其排卵。在排卵时间附近进行与可育雄性的自然交配或人工授精。注射HCG后36小时和11天，对雌性进行腹腔镜检查以确定是否发生排卵。在腹腔镜控制下，在注射HCG后4至7天和15至17天，用1.2至3毫升温热盐水冲洗子宫腔。通过插入清洁阴道的聚乙烯导管或巴斯德吸管回收冲洗液。共进行了58次子宫冲洗，平均液体回收率为65.4%。通过该技术回收了6个未受精卵和2个植入前囊胚。
