Qian M, Gallo J M
Department of Pharmaceutics, College of Pharmacy, University of Georgia, Athens 30602.
J Chromatogr. 1992 Jul 24;578(2):316-20. doi: 10.1016/0378-4347(92)80432-p.
A new high-performance liquid chromatographic (HPLC) assay was developed for the determination of nimodipine in monkey plasma. An ethyl acetate extraction procedure was employed with a reversed-phase HPLC separation for the analysis. Absolute recovery of nimodipine from plasma was over 95% with a lower limit of quantitation of 10 ng/ml. This method was applied to a preliminary pharmacokinetic study in which 0.25 mg/kg nimodipine was administered intravenously to three monkeys. Protein binding and stability of nimodipine in monkey plasma were also examined. The pharmacokinetic parameters of nimodipine in monkeys were similar to those obtained in humans and indicate that monkeys are an appropriate animal model for further pharmacokinetic investigations.
开发了一种新的高效液相色谱（HPLC）分析法，用于测定猴血浆中的尼莫地平。采用乙酸乙酯萃取法，并通过反相HPLC分离进行分析。尼莫地平从血浆中的绝对回收率超过95%，定量下限为10 ng/ml。该方法应用于一项初步药代动力学研究，其中向三只猴子静脉注射0.25 mg/kg的尼莫地平。还检测了尼莫地平在猴血浆中的蛋白结合率和稳定性。尼莫地平在猴子体内的药代动力学参数与在人体中获得的参数相似，表明猴子是进一步进行药代动力学研究的合适动物模型。
