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鼠源性卡氏肺孢子虫对培养的水貂肺细胞（MiCl1）垂直单层细胞的黏附作用。

Murine Pneumocystis carinii adherence to vertical monolayers of cultured mink lung cells (MiCl1).

作者信息

Garner R E, Walker A N, Horst M N

机构信息

Department of Basic Medical Sciences, Mercer University School of Medicine, Macon, Georgia 31207.

出版信息

J Clin Microbiol. 1992 Sep;30(9):2467-70. doi: 10.1128/jcm.30.9.2467-2470.1992.

DOI:10.1128/jcm.30.9.2467-2470.1992
PMID:1401017
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265525/
Abstract

We describe a method for adherence and culture of murine Pneumocystis carinii in mink lung cells (MiCl1) grown on vertical supports. The vertical cultures were infected with P. carinii; the surrounding medium and inoculum were stirred to ensure circulation and contact with MiCl1 cells. When compared with conventional horizontal culture, the vertical method offers a more suitable system for assessing P. carinii adherence. This approach has proved suitable for quantitative evaluation of P. carinii adherence to MiCl1 cells in the presence of inhibitors.

摘要

我们描述了一种在垂直支架上生长的水貂肺细胞（MiCl1）中对鼠卡氏肺孢子虫进行黏附及培养的方法。垂直培养物用卡氏肺孢子虫感染；搅拌周围的培养基和接种物以确保循环并与MiCl1细胞接触。与传统的水平培养相比，垂直方法为评估卡氏肺孢子虫黏附提供了更合适的系统。这种方法已被证明适用于在存在抑制剂的情况下对卡氏肺孢子虫黏附于MiCl1细胞进行定量评估。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73d5/265525/f5de0fbffe0f/jcm00033-0267-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73d5/265525/a1f57a2c0d6d/jcm00033-0266-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73d5/265525/ded70b31a22e/jcm00033-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73d5/265525/f5de0fbffe0f/jcm00033-0267-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73d5/265525/a1f57a2c0d6d/jcm00033-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73d5/265525/8f6b81b5007f/jcm00033-0266-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73d5/265525/ded70b31a22e/jcm00033-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73d5/265525/f5de0fbffe0f/jcm00033-0267-b.jpg

相似文献

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Murine Pneumocystis carinii adherence to vertical monolayers of cultured mink lung cells (MiCl1).鼠源性卡氏肺孢子虫对培养的水貂肺细胞（MiCl1）垂直单层细胞的黏附作用。
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引用本文的文献

1
Continuous axenic cultivation of Pneumocystis carinii.卡氏肺孢子虫的连续无菌培养
Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2402-7. doi: 10.1073/pnas.96.5.2402.

本文引用的文献

1
Growth and serial passage of Pneumocystis carinii in the A549 cell line.卡氏肺孢子虫在A549细胞系中的生长及连续传代
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Attachment of microbes to host cells: relevance of Pneumocystis carinii.微生物与宿主细胞的附着：卡氏肺孢子虫的相关性
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Growth and metabolism of Pneumocystis carinii in axenic culture.卡氏肺孢子虫在无细胞培养中的生长与代谢
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Morphologic and biochemical studies of chitin expression in Pneumocystis carinii.卡氏肺孢子虫中几丁质表达的形态学和生化研究。
J Protozool. 1991 Nov-Dec;38(6):12S-14S.
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Modulation of Pneumocystis carinii adherence to cultured lung cells by a mannose-dependent mechanism.通过一种甘露糖依赖性机制调节卡氏肺孢子虫对培养肺细胞的黏附。
J Lab Clin Med. 1991 Nov;118(5):492-9.
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The interaction in vitro of Pneumocystis carinii with macrophages and L-cells.卡氏肺孢子虫与巨噬细胞和L细胞的体外相互作用。
J Exp Med. 1978 Jan 1;147(1):157-70. doi: 10.1084/jem.147.1.157.
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Cultivation of Pneumocystis carinii with WI-38 cells.用WI-38细胞培养卡氏肺孢子菌。
J Clin Microbiol. 1979 Dec;10(6):796-9. doi: 10.1128/jcm.10.6.796-799.1979.
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Pneumocystis carinii: new separation method from lung tissue.卡氏肺孢子虫：从肺组织中分离的新方法。
Exp Parasitol. 1979 Jun;47(3):356-68. doi: 10.1016/0014-4894(79)90088-2.