Koppang H S, Koppang R, Stolen S O
Dental Faculty, University of Oslo, Norway.
J Dent Assoc S Afr. 1992 May;47(5):210-6.
The present investigation was undertaken to identify commonly occurring foreign material in postendodontic periapical granulomas and cysts. 29 biopsies from such lesions with observed foreign material were routinely processed, stained with H&E, von Kossa and Calcofluor White and investigated by light, polarization and fluorescence microscopy. Applying back-scattered SEM images, the foreign material was subjected to energy dispersive X-ray analysis. 4 groups of foreign material were observed: 1. Black/brownish fragments and yellow/brown granules containing Au, Ag, Cu, Hg, Sn and Zn compatible with amalgam. 2. Fine black/brown/yellow granules compatible with endodontic sealer components revealing Ag, Ba, Bi, Cu, S, Ti and Zn. 3. Basophilic fragments compatible with Ca salts from Ca(OH), extruded periapically and containing Ca and P. 4. Elongated/rounded/oval/kidney-shaped, colourless to slightly basophilic, birefringent structures revealing C and O and with a slit-like central canal and a bright, pale-blue fluorescence specific for cellulose.
本研究旨在确定根管治疗后根尖周肉芽肿和囊肿中常见的外来物质。对29例观察到有外来物质的此类病变组织进行活检，常规处理后用苏木精-伊红染色、冯·科萨染色和碱性蕊香红荧光白染色，并通过光学显微镜、偏光显微镜和荧光显微镜进行观察。应用背散射扫描电子显微镜图像，对异物进行能量色散X射线分析。观察到4组外来物质：1. 黑色/褐色碎片和黄色/褐色颗粒，含有与汞合金相符的金、银、铜、汞、锡和锌。2. 与根管封闭剂成分相符的细小黑色/褐色/黄色颗粒，含有银、钡、铋、铜、硫、钛和锌。3. 与来自氢氧化钙的钙盐相符的嗜碱性碎片，根尖周挤出，含有钙和磷。4. 细长/圆形/椭圆形/肾形，无色至微嗜碱性，双折射结构，含有碳和氧，有一条狭缝状中央管，发出纤维素特有的明亮淡蓝色荧光。
