Young W G, Zhang C Z, Li H, Osborne P, Waters M J
Division of Oral Biology & Pathology, Faculty of Dentistry, University of Queensland, Australia.
J Dent Res. 1992 Nov;71(11):1807-11. doi: 10.1177/00220345920710110801.
For investigation of how growth hormone affects tooth development, bromodeoxyuridine immunocytochemistry and morphometry were used for the study of cell proliferation in odontogenic epithelial cell layers. The number of cells in the S phase, as revealed by this technique, and in mitosis, were counted in Bouin's-perfused and paraffin-embedded undecalcified maxillary incisor enamel organs of normal rats, in growth-hormone-deficient dwarf rats, and in dwarf rats treated with growth hormone (66 micrograms/100 g body wt) twice daily for six days. Significantly fewer labeled nuclei, unlabeled nuclei, and total nuclei of various odontogenic epithelia were found in dwarf rats, but in dwarf rats treated with growth hormone, numbers of labeled nuclei equivalent to normal were found in the internal enamel epithelium, stratum intermedium, and Hertwig root sheath. Moreover, the mitotic index for pre-ameloblasts was 1.64 in normal rats, 0.92 for dwarf rats, and 1.66 for growth-hormone-treated dwarf rats (SD, 0.10). Other parameters--such as the labeling index and the ratio of positive to negative nuclei--were similarly related to GH status. Thus, growth hormone may play a role in the proliferation of the odontogenic epithelia in the rat.
为研究生长激素如何影响牙齿发育，采用溴脱氧尿苷免疫细胞化学和形态计量学方法，对牙源性上皮细胞层中的细胞增殖进行研究。通过该技术揭示的处于S期和有丝分裂期的细胞数量，在正常大鼠、生长激素缺乏的侏儒大鼠以及每天两次（连续6天）接受生长激素治疗（66微克/100克体重）的侏儒大鼠的经Bouin液灌注并石蜡包埋的未脱钙上颌切牙釉器中进行计数。在侏儒大鼠中，各种牙源性上皮的标记核、未标记核和总核数量明显减少，但在接受生长激素治疗的侏儒大鼠中，内釉上皮、中间层和赫特维希根鞘中的标记核数量与正常大鼠相当。此外，正常大鼠中成釉细胞前体细胞的有丝分裂指数为1.64，侏儒大鼠为0.92，接受生长激素治疗的侏儒大鼠为1.66（标准差，0.10）。其他参数，如标记指数以及阳性核与阴性核的比例，同样与生长激素状态相关。因此，生长激素可能在大鼠牙源性上皮的增殖中发挥作用。
