BROOKS L D, NORTHEY W T
J Bacteriol. 1963 Jan;85(1):12-5. doi: 10.1128/jb.85.1.12-15.1963.
Brooks, Lula D. (Arizona State University, Tempe) and William T. Northey. Studies on Coccidioides immitis. II. Physiological studies on in vitro spherulation. J. Bacteriol. 85:12-15. 1963.-Studies on the amino acids assayed in Edamine revealed that the amino acids phenylalanine, tyrosine, and tryptophan, in addition to the amino acid derivatives dihydroxyphenylalanine (DOPA), epinephrine, tyramine monohydrochloride (tyramine HCl), and pyrocatechol play an important role in the Coccidioides immitis conversion process. In the presence of phenylalanine, tyrosine, tryptophan, DOPA, pyrocatechol, dextrose, and biotin, conversion as well as maturation was demonstrated. However, in epinephrine and tyramine HCl combinations, conversion occurred without maturation of the spherules. The availability of increased amounts of melanin precursors is suggested as a possible explanation for the increased susceptibility of the dark-skinned races to disseminated coccidioidomycosis. Conversion and maturation were also noted when lactic and fumaric acids served as carbon sources. The conversion of a limited number of arthrospores to spherules and their subsequent maturation in the presence of only phenol and biotin suggest the possibility that the key to the C. immitis conversion process lies in the availability of certain ring structures.
布鲁克斯,卢拉·D.(亚利桑那州立大学,坦佩)和威廉·T.诺西。粗球孢子菌研究。二。体外球状体形成的生理学研究。《细菌学杂志》85:12 - 15。1963年。——对伊登明中检测的氨基酸的研究表明,除了氨基酸衍生物二羟基苯丙氨酸(多巴)、肾上腺素、盐酸酪胺(酪胺盐酸盐)和邻苯二酚外,苯丙氨酸、酪氨酸和色氨酸在粗球孢子菌的转化过程中起重要作用。在苯丙氨酸、酪氨酸、色氨酸、多巴、邻苯二酚、葡萄糖和生物素存在的情况下,证明了转化以及成熟过程。然而,在肾上腺素和酪胺盐酸盐组合中,发生了转化但球状体未成熟。黑色素前体数量增加的可利用性被认为是黑皮肤种族对播散性球孢子菌病易感性增加的一种可能解释。当乳酸和富马酸作为碳源时,也观察到了转化和成熟。在仅存在苯酚和生物素的情况下,有限数量的关节孢子转化为球状体并随后成熟,这表明粗球孢子菌转化过程的关键可能在于某些环状结构的可利用性。