Heyborne K D, Cranfill R L, Carding S R, Born W K, O'Brien R L
Department of Obstetrics and Gynecology, University of Colorado Health Sciences Center, Denver.
J Immunol. 1992 Nov 1;149(9):2872-8.
A specific subset of gamma delta T lymphocytes bearing a V gamma 6V delta 1-encoded TCR is known to populate the normal nonpregnant murine uterine and vaginal epithelium. However, gamma delta T lymphocytes residing at the maternal-fetal interface during pregnancy have not yet been investigated. Using mAb and cytofluorographic analysis, we analyzed gamma delta TCR-bearing lymphocytes obtained from placental/decidual tissues of allogeneic (C57B1/10 X BALB/c and BALB/c X C57B1/10) pregnancies. Gestations were analyzed at several time points during the second half of pregnancy, with lymphocytes from both maternal spleen and nonpregnant C57B1/10 uteri analyzed as controls. We found that all gamma delta T lymphocytes at the maternal-fetal interface are maternally derived. Relative to the total T lymphocyte population, the percentage of gamma delta TCR-bearing T lymphocytes at the maternal-fetal interface is enriched three- to four-fold compared with maternal spleen, and twofold compared with nonpregnant uteri. Although one-third of gamma delta T lymphocytes from pregnant animals express a cell-surface marker associated with activation (IL-2R), gamma delta cells from uteri of nonpregnant mice fail to express IL-2R. In terms of absolute numbers, we estimate that reproductive-tract gamma delta T cells are increased nearly 100-fold in pregnant animals compared with nonpregnant animals. To characterize the TCR-gamma delta repertoire in the placenta/decidua, we generated 21 TCR-gamma delta-bearing hybridomas from lymphocytes in this tissue. Analysis of these hybridomas revealed at least six distinct gamma delta receptor types expressed at the maternal-fetal interface, with V gamma 6V delta 1 encoded TCR representing the predominant population. As specific resident constituents of the reproductive tract, gamma delta T lymphocytes may be involved in regulating a variety of physiologic and pathophysiologic events in reproductive biology.
已知带有Vγ6Vδ1编码的TCR的γδT淋巴细胞的一个特定亚群存在于正常未孕小鼠的子宫和阴道上皮中。然而，孕期位于母胎界面的γδT淋巴细胞尚未得到研究。我们使用单克隆抗体和细胞荧光分析，对从同种异体（C57B1/10×BALB/c和BALB/c×C57B1/10）妊娠的胎盘/蜕膜组织中获得的带有γδTCR的淋巴细胞进行了分析。在妊娠后半期的几个时间点对妊娠情况进行了分析，同时分析了来自母体脾脏和未孕C57B1/10子宫的淋巴细胞作为对照。我们发现，母胎界面处所有的γδT淋巴细胞均来自母体。相对于总的T淋巴细胞群体，母胎界面处带有γδTCR的T淋巴细胞的百分比与母体脾脏相比增加了三到四倍，与未孕子宫相比增加了两倍。尽管来自妊娠动物的γδT淋巴细胞中有三分之一表达与活化相关的细胞表面标志物（IL-2R），但来自未孕小鼠子宫的γδ细胞不表达IL-2R。就绝对数量而言，我们估计妊娠动物生殖道γδT细胞的数量比未孕动物增加了近100倍。为了表征胎盘/蜕膜中的TCR-γδ库，我们从该组织中的淋巴细胞产生了21个带有TCR-γδ的杂交瘤。对这些杂交瘤的分析显示，母胎界面处至少表达六种不同的γδ受体类型，其中Vγ6Vδ1编码的TCR占主要群体。作为生殖道的特定常驻成分，γδT淋巴细胞可能参与调节生殖生物学中的各种生理和病理生理事件。
