重组鼠肿瘤坏死因子（TNF）抗体可中和豚鼠TNF。

Antibody to recombinant murine tumor necrosis factor (TNF) neutralizes guinea pig TNF.

作者信息

Ruppel-Kerr R, Lemp J A, Karol M H

机构信息

Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, PA 15261.

出版信息

J Immunol Methods. 1992 Oct 2;154(2):179-84. doi: 10.1016/0022-1759(92)90190-5.

DOI:10.1016/0022-1759(92)90190-5

PMID:1401952

Abstract

Cotton dust has been found to cause acute pulmonary inflammation and fever in humans and in a guinea pig model of byssinosis. Following 3 h inhalation of cotton dust particles, guinea pig macrophages were found to release ex vivo a factor(s) toxic to WEHI fibrosarcoma cells. The cytotoxic factor(s) was also present in the bronchoalveolar fluid. We sought to investigate the mechanism of the inflammatory response to determine whether the factor was TNF. Antibodies to murine TNF were produced by immunizing sets of rabbits using two protocols. All animals produced anti-TNF antibodies with titers of 1/1000-1/25,000. Sera from one set of animals completely neutralized the cytotoxicity of murine TNF toward the WEHI cell line. The antisera neutralized up to 93% of the cytotoxicity of guinea pig samples but only 54% of human recombinant TNF. These results identify TNF in pulmonary tissues of guinea pigs following exposure to cotton dust. Moreover, the studies indicate that rabbit antibodies to murine TNF can be used to detect the guinea pig cytokine.

摘要

在人类以及棉尘肺的豚鼠模型中，已发现棉尘可导致急性肺部炎症和发热。在吸入棉尘颗粒3小时后，发现豚鼠巨噬细胞在体外释放出一种对WEHI纤维肉瘤细胞有毒的因子。支气管肺泡灌洗液中也存在这种细胞毒性因子。我们试图研究炎症反应的机制，以确定该因子是否为肿瘤坏死因子（TNF）。通过两种方案免疫多组兔子，制备了抗小鼠TNF的抗体。所有动物均产生了滴度为1/1000 - 1/25000的抗TNF抗体。一组动物的血清完全中和了小鼠TNF对WEHI细胞系的细胞毒性。这些抗血清可中和高达93%的豚鼠样本的细胞毒性，但只能中和54%的人重组TNF。这些结果确定了豚鼠暴露于棉尘后肺组织中的TNF。此外，研究表明，抗小鼠TNF的兔抗体可用于检测豚鼠细胞因子。