CHANG S L, HALL H E
Appl Microbiol. 1963 Jul;11(4):365-7. doi: 10.1128/am.11.4.365-367.1963.
The usefulness of free-living nematodes for assaying staphylococcal enterotoxin was evaluated with a 98% pure enterotoxin B on five different nematodes. Included in the evaluation was an enterotoxin B in a crude culture filtrate. The filtrate of a culture of nonenterotoxigenic strains of Staphylococcus aureus, the uninoculated respective broth media, and distilled water were used as controls. The purified enterotoxin was found to exert no toxic effects at dosages ranging from 10 to 1,000 mug/ml for as long as 24 hr. Utilization of the toxin-protein by these nematodes was evidenced by their propagation after exposure times longer than 24 hr. The crude filtrate, containing 28 mug of enterotoxin per ml, was detrimental to nematodes to the same degree as the nontoxic filtrate and the uninoculated broths, in that they all caused irritation to external genitalia, motility changes, and death after comparable exposure times. This is in agreement with earlier observations that standard bacteriological fluid media, or broths containing over 1% protein hydrolysate or 1 to 2% salts, exert toxic effects on free-living nematodes.
用98%纯度的肠毒素B对五种不同的线虫评估了自由生活线虫用于检测葡萄球菌肠毒素的效用。评估中包括粗培养滤液中的肠毒素B。金黄色葡萄球菌非产肠毒素菌株培养物的滤液、未接种的相应肉汤培养基和蒸馏水用作对照。发现纯化的肠毒素在剂量为10至1000微克/毫升、长达24小时的情况下没有毒性作用。暴露时间超过24小时后线虫的繁殖证明了这些线虫对毒素蛋白的利用。每毫升含有28微克肠毒素的粗滤液对线虫的有害程度与无毒滤液和未接种的肉汤相同，因为在相当的暴露时间后，它们都会导致外生殖器刺激、运动变化和死亡。这与早期的观察结果一致，即标准细菌液体培养基或含有超过1%蛋白水解物或1至2%盐的肉汤对自由生活线虫有毒性作用。
