Kageyama K, Onoyama Y, Matsui-Yuasa I, Otani S, Morisawa S, Kanayama Y, Takeda T
Radioisotope Center, Osaka City University Medical School, Japan.
Int J Hyperthermia. 1992 Jul-Aug;8(4):495-9. doi: 10.3109/02656739209037987.
Effect of hyperthermia and/or protein kinase inhibitors on DNA synthesis and cell proliferation was investigated in Ehrlich ascites tumour cells in vitro. Both H-7 and H-8, potent inhibitors of protein kinase C, suppressed DNA synthesis significantly, but HA1004, an inhibitor of cAMP- and cGMP-dependent protein kinase, did not. Hyperthermia increased greatly the suppressive activity of H-7 and H-8 but not that of HA1004. H-7 also inhibited cell growth. These results suggest that the inhibition of protein kinase C enhances the suppression of DNA synthesis and the proliferation of tumour cells by hyperthermia.
在体外对艾氏腹水瘤细胞研究了热疗和/或蛋白激酶抑制剂对DNA合成及细胞增殖的影响。蛋白激酶C的强效抑制剂H-7和H-8均显著抑制DNA合成，但cAMP和cGMP依赖性蛋白激酶的抑制剂HA1004则无此作用。热疗极大地增强了H-7和H-8的抑制活性，但未增强HA1004的抑制活性。H-7也抑制细胞生长。这些结果提示，抑制蛋白激酶C可增强热疗对肿瘤细胞DNA合成及增殖的抑制作用。
