Martinez-Padrón M, González G C, Lederis K, Lukowiak K
Department of Physiology, Faculty of Medicine, University of Calgary, Alberta, Canada.
J Neurocytol. 1992 Sep;21(9):672-8. doi: 10.1007/BF01191728.
Chromatographic and immunological evidence indicates that a vasopressin-like peptide might be present in the CNS of Aplysia californica, and that this peptide may be involved in modulating the behaviour of the gill. Immunocytochemical techniques using antisera raised against various vasopressin-like peptides were used to localize the sites containing these peptides in the CNS of Aplysia. Vasopressin-like immunoreactivity was found to be restricted to one single neuron in the abdominal ganglion and two small neurons located bilaterally in each pedal ganglion. Immunoreactive fibres were present in the neuropile of the abdominal, pedal, pleural and cerebral ganglia, but not in the buccal ganglion. The identification of these neurons provides a morphological localization for vasopressin-like substances detected previously in CNS extracts of Aplysia californica. In addition, the possibility of electrophysiological studies involving the immunoreactive neurons identified in the present paper will allow a more direct approach to study the physiological role of vasopressin-like peptides in Aplysia.
色谱分析和免疫学证据表明，加利福尼亚海兔的中枢神经系统中可能存在一种类似加压素的肽，并且这种肽可能参与调节鳃的行为。使用针对各种类似加压素的肽产生的抗血清的免疫细胞化学技术，用于在加利福尼亚海兔的中枢神经系统中定位含有这些肽的部位。发现类似加压素的免疫反应性仅限于腹神经节中的一个单一神经元以及位于每个足神经节两侧的两个小神经元。免疫反应性纤维存在于腹神经节、足神经节、胸膜神经节和脑神经节的神经纤维网中，但不存在于口神经节中。这些神经元的鉴定为先前在加利福尼亚海兔中枢神经系统提取物中检测到的类似加压素的物质提供了形态学定位。此外，涉及本文中鉴定的免疫反应性神经元的电生理学研究的可能性，将允许采用更直接的方法来研究类似加压素的肽在加利福尼亚海兔中的生理作用。
