Longley R D, Longley A J
J Neurobiol. 1986 Jul;17(4):339-58. doi: 10.1002/neu.480170408.
Serotonergic neurons and axons were mapped in the central ganglia of Aplysia californica using antiserotonin antibody on intact ganglia and on serial sections. Immunoreactive axons and processes were present in all ganglia and nerves, and distinct somata were detected in all ganglia except the buccal and pleural ganglia. The cells stained included known serotonergic neurons: the giant cerebral neurons and the RB cells of the abdominal ganglion. The area of the abdominal ganglion where interneurons are located which produce facilitation during the gill withdrawal reflex was carefully examined for antiserotonin immunoreactive neurons. None were found, but two bilaterally symmetric pairs of immunoreactive axons were identified which descend from the contralateral cerebral or pedal ganglion to abdominal ganglion. Because of the continuous proximity of this pair of axons, they could be recognized and traced into the abdominal ganglion neuropil in each preparation. If serotonin is a facilitating transmitter in the abdominal ganglion, these and other antiserotonin immunoreactive axons in the pleuroabdominal connectives may be implicated in this facilitation.
使用抗血清素抗体，在完整神经节和连续切片上对加州海兔中枢神经节中的血清素能神经元和轴突进行了定位。免疫反应性轴突和突起存在于所有神经节和神经中，除了口神经节和胸膜神经节外，在所有神经节中都检测到了不同的胞体。染色的细胞包括已知的血清素能神经元：巨大脑神经元和腹神经节的RB细胞。仔细检查了腹神经节中在鳃收缩反射期间产生易化作用的中间神经元所在区域，以寻找抗血清素免疫反应性神经元。未发现此类神经元，但鉴定出两对双侧对称的免疫反应性轴突，它们从对侧脑或足神经节下行至腹神经节。由于这对轴突持续相邻，在每个标本中都能识别并追踪它们进入腹神经节神经纤维网。如果血清素是腹神经节中的易化性递质，那么胸膜腹神经连接中这对轴突和其他抗血清素免疫反应性轴突可能与这种易化作用有关。
