Sevall J S, Ritenhous J, Peter J B
Specialty Laboratories, Inc., Santa Monica, California.
J Clin Lab Anal. 1992;6(4):171-5. doi: 10.1002/jcla.1860060402.
The sensitivity and application of the polymerase chain reaction (PCR) for the diagnosis of parvovirus B19 (B19) infection was investigated by simultaneously assaying a collection of 279 consecutively received samples for presence of anti-B19 IgM and IgG antibodies by Western blot and for B19 DNA by PCR and dot-blot hybridization (dot-blot); samples were sera from patients with suspected B19 infection. PCR and dot-blot detected B19 DNA in 9% (16/179) and 1% (2/179), respectively of Ab-positive samples (IgM+/IgG-, IgM+IgG+, IgM-IgG+), and in 28% (15/54) and 2% (1/54), respectively, of IgM+ samples. PCR also detected B19 DNA in 2% (2/100) of IgM-/IgG- samples, both of which had normal total IgG and IgM levels. PCR is of unique value because it permits diagnosis of B19 infection even in the absence of specific acute phase (IgM) and in the presence or absence of convalescent-phase (IgG) Ab.
通过对连续收集的279份样本同时进行检测，研究了聚合酶链反应（PCR）诊断微小病毒B19（B19）感染的敏感性和应用情况。这些样本均来自疑似B19感染患者的血清，采用蛋白质印迹法检测抗B19 IgM和IgG抗体，采用PCR和斑点印迹杂交（斑点印迹）法检测B19 DNA。PCR和斑点印迹分别在9%（16/179）和1%（2/179）的抗体阳性样本（IgM+/IgG-、IgM+IgG+、IgM-IgG+）中检测到B19 DNA，在IgM+样本中分别为28%（15/54）和2%（1/54）。PCR还在2%（2/100）的IgM-/IgG-样本中检测到B19 DNA，这两份样本的总IgG和IgM水平均正常。PCR具有独特的价值，因为即使在没有特异性急性期（IgM）抗体以及存在或不存在恢复期（IgG）抗体的情况下，它也能诊断B19感染。
