Shaw P A, Pringle J H
Department of Histopathology, Leicester Royal Infirmary, U.K.
J Pathol. 1992 Aug;167(4):375-80. doi: 10.1002/path.1711670405.
Previous studies using immunohistochemistry have shown variable hormone production by carcinoid tumours of the appendix. In order to confirm the existence of a specific subset of these tumours, in situ hybridization using synthetic oligonucleotide probes to detect pre-proglucagon and pre-proinsulin mRNA was performed in formalin-fixed, paraffin-embedded material from eight tubular carcinoids, 12 insulin carcinoids, and two mucinous carcinoids. The results were correlated with standard silver and mucin stains. All tubular carcinoids but none of the insular or mucinous carcinoids contained proglucagon mRNA. Proinsulin mRNA was not detected in any of the tumours. Tubular carcinoids of the appendix constitute a definable subset of appendiceal carcinoids which have a similar distribution and prognosis to typical insular carcinoids and can be diagnosed on haematoxylin and eosin-stained sections confirmed by routine special stains. The main need for recognition is to avoid confusion with mucinous carcinoids, which have a worse prognosis and may require more aggressive treatment.
以往使用免疫组织化学的研究表明，阑尾类癌肿瘤产生的激素各不相同。为了证实这些肿瘤中特定亚群的存在，我们使用合成寡核苷酸探针进行原位杂交，以检测8例管状类癌、12例胰岛素类癌和2例黏液性类癌的福尔马林固定、石蜡包埋材料中的前胰高血糖素原和前胰岛素原mRNA。结果与标准银染和黏液染色相关。所有管状类癌均含有胰高血糖素原mRNA，而岛状或黏液性类癌均未检测到。在任何肿瘤中均未检测到胰岛素原mRNA。阑尾管状类癌构成阑尾类癌的一个可定义亚群，其分布和预后与典型岛状类癌相似，可通过苏木精和伊红染色切片并经常规特殊染色证实进行诊断。识别的主要需求是避免与黏液性类癌混淆，黏液性类癌预后较差，可能需要更积极的治疗。
