Coleman J J, Siwy B K
Department of Surgery, Indiana University Medical Center, Indianapolis.
J Pediatr Surg. 1992 Aug;27(8):1029-32. doi: 10.1016/0022-3468(92)90552-i.
The recent more widespread availability of cultured keratinocytes has increased their clinical applicability in pediatric surgical problems. In life threatening burns (3 patients) and in elective procedures (3 patients) we have used sheets of cultured epithelial autografts. Body surface area affected ranged from fifteen to 85%. Five patients were successfully treated in one grafting session and one required four sessions. Take of the cultured keratinocytes ranged from 75% to 95%. Follow-up as long as 30 months has shown the skin to be comparable to if not superior to conventional split thickness grafts. The use of cultured keratinocytes allows wound coverage without expansion of the affected body surface area seen with conventional split-thickness skin grafting. Although there is an initial delay in the preparation of the grafts, the overall results have been excellent and suggest the more frequent use of this method.
最近，培养的角质形成细胞更广泛可得，这增加了它们在小儿外科问题中的临床适用性。在危及生命的烧伤患者（3例）和择期手术患者（3例）中，我们使用了培养的上皮自体移植片。受影响的体表面积范围为15%至85%。5例患者在一次移植手术中得到成功治疗，1例需要4次手术。培养的角质形成细胞的成活率在75%至95%之间。长达30个月的随访表明，移植的皮肤即便不比传统的中厚皮片优越，至少也与之相当。使用培养的角质形成细胞可实现伤口覆盖，而不会像传统中厚皮片移植那样导致受影响的体表面积扩大。尽管在制备移植片时有初始延迟，但总体结果非常出色，表明该方法可更频繁使用。
