Weems C W, Weems Y S, Lee C N, Vincent D L
Department of Animal Sciences, University of Hawaii at Manoa, Honolulu 96822.
Second Messengers Phosphoproteins. 1992;14(1-2):1-9.
In two separate experiments, control and PGF2 alpha-treated unilaterally ovariectomized ewes received vehicle or adenosine every four hours through an exteriorized catheter installed either to parafuse the ovarian vascular pedicle of the remaining ovary containing a corpus luteum (CL) or to infuse intrauterine ipsilateral to the remaining ovary with the CL. Infusions were given every four hours from day 7 through day 22 postestrus or until ewes returned to estrus. Chronic infusion of vehicle intrauterine or parafusion of the ovarian vascular pedicle with vehicle in PGF2 alpha-treated ewes shortened the interestrous interval compared to controls (P less than 0.05). However, chronic parafusion of the ovarian vascular pedicle with adenosine in PGF2 alpha-treated ewes prevented a PGF2 alpha-induced premature luteolysis (P less than 0.05) but not when adenosine was infused chronically intrauterine (P greater than 0.05). It is concluded that adenosine can block a PGF2 alpha-induced premature luteolysis in vivo and may have roles in regulation of luteal secretion of progesterone but it is probably not the embryonic antiluteolysin of early pregnancy in ewes.
在两项独立实验中，对照组和接受前列腺素F2α（PGF2α）处理的单侧卵巢切除母羊，通过安装的外置导管每四小时接受一次赋形剂或腺苷，该导管要么用于对含有黄体（CL）的剩余卵巢的卵巢血管蒂进行体外灌注，要么用于向同侧含有CL的剩余卵巢的子宫内注入。从发情期后第7天到第22天或直到母羊恢复发情，每四小时进行一次输注。与对照组相比，在接受PGF2α处理的母羊中，子宫内长期输注赋形剂或用赋形剂对卵巢血管蒂进行体外灌注缩短了发情间期（P<0.05）。然而，在接受PGF2α处理的母羊中，用腺苷对卵巢血管蒂进行长期体外灌注可防止PGF2α诱导的过早黄体溶解（P<0.05），但当腺苷在子宫内长期注入时则不能（P>0.05）。得出的结论是，腺苷可在体内阻断PGF2α诱导的过早黄体溶解，可能在调节黄体分泌孕酮方面发挥作用，但它可能不是母羊早期妊娠的胚胎抗黄体溶解素。
